Publications

2009

• Varadarajan N., Pogson, M., Georgiou G. and B.L. Iverson, “Proteases that Can Distinguish Among Different Post-Translational Forms of Tyrosine Engineered using Multi-color Flow Cytometry" J. Amer. Chem. Soc. Epub (2009) PMID: 19924991 
• Cantor, J.R., Stone, E.M., Chantranupong, L. and G. Georgiou, “The Human Asparaginase-like Protein hASRGL1 is an Ntn Hydrolase and the First Mammalian Member of the β-Aspartyl Peptidase Family,” Biochemistry 48:11026-31 (2009) PMID: 19839645
• Skretas, G. Carroll S., DeFrees, S., Schwartz, M.F. Johnson, K.F. and G. Georgiou, “Expression of an Active Human Glycosyltransferase (Sialyltransferase ST6GalNAcI) in Escherichia coli Strains with Oxidizing Cytoplasm,”  Microb. Cell Factories 8:50 (2009) PMID: 19788761
• Pogson, M., Georgiou, G. and B.L. Iverson, “Engineering Next Generation Proteases,” Curr Opin Biotechnol. 20:390-7 (2009) PMID: 19709875
• Arredondo SA, Chen TF, Riggs AF, Gilbert HF, Georgiou G. "The Role of Dimerization on the Catalytic Properties of the Escherichia coli Disulfide Isomerase DsbC," J Biol Chem. 284:23972-9 (2009). PMID: 19581640
• Zhou, L., Zhao, M., Wolf, R.Z,, Graham, D.E. and Georgiou G.,” Transcriptional Regulation of the Escherichia coli rraB, Encoding a Protein Inhibitor of RNase E”  J. Bacteriol. 191:6665-74 (2009) PMID: 19709875
• Pogson, M., Georgiou, G. and B.L. Iverson, “Engineering next generation proteases,” Curr Opin Biotechnol. 2009 (Epub ahead of print) PMID: 19709875
• Arredondo SA, Chen TF, Riggs AF, Gilbert HF, Georgiou G. "The role of dimerization on the catalytic properties of the Escherichia coli disulfide isomerase DsbC," J Biol Chem. (2009 Jul 6) Epub ahead of print PMID: 19581640
• Mazor Y, Van Blarcom T, Iverson BL, Georgiou G. “Isolation of full-length IgG antibodies from combinatorial libraries expressed in Escherichia coli,” Methods Mol Biol. 525:217-39, xiv (2009) PMID: 19252859
• Varadarajan, N., Cantor, J., Georgiou, G. and B.L. Iverson, “Construction and flow cytometric screening of targeted enzyme libraries” Nature Protocol 4:893-901 (2009) PMID: 19478805 pdf
• Leysath, C.E., Monzingo, A.F., Maynard, J.A., Barnett, J., Georgiou, G., Iverson, B.L. and Robertus, J.D. “Crystal Structure of the Engineered Neutralizing Antibody M18 Complexed to the Anthrax Protective Antigen” J Mol Biol. 3:387(3):680-93 (2009) PMCID: PMC2670345 pdf
• Eser, M.,  Masip, L.,  Kadokura, H. Georgiou, G. and J.Beckwith, “Disulfide bond formation by exported glutaredoxin indicates glutathione's presence in the E. coli periplasm.,” Proc Natl Acad Sci U S A 106(5):1571-7 (2009) PMCID: PMC2635786 pdf
• Seo, M.-J., Jeong K-J., Leysath, C.E., Ellington, A.D., Iverson, B.L. and G. Georgiou, “Engineering Antibody Fragments to Fold in the Absence of Disulfide Bonds,” Prot Sci. 18:259-67 (2009). PMCID: PMC2635786 pdf
• Skretas G. and G. Georgiou, “Genetic Analysis of G Protein-Coupled Receptor Expression in Escherichia coli: Inhibitory Role of DnaJ on the Membrane Integration of the Human Central Cannabinoid Receptor,”  Biotechnol Bioeng. 1;102(2):357-67(2009) PMCID: PMC2666920 

2008

• Mazor, Y., Van Blarcom T., Iverson, B.L. and G. Georgiou, “E-clonal antibodies: selection of Full-Length IgG Antibodies Using Bacterial Periplasmic Display” Nature Protocol. 3:1766-77 (2008) PMID 18948976. pdf
• Arredondo, S., Segatori L., Gilbert H.G. and G. Georgiou, “De novo Engineering and Evolution of Disulfide Isomerases Analogous to the Bacterial DsbC,” J. Biol. Chem 283(46):31469-76 PMCID: PMC2581571 (2008) pdf
• Varadarajan, N., Cantor, JR, Georgiou G. and B.L. Iverson, “Engineering a Protease that Cleaves after Sulfotyrosine,” Angew. Chem Intl Ed. 47(41):7861-3 (2008) PMCID: PMC2671331 pdf
• Skretas, G. and G. Georgiou, “Engineering G Protein-Coupled Receptor Expression in Bacteria, “ Proc Natl Acad Sci U S A. 105:14747-8. (2008). PMID: 18812517 pdf
• Link, J.A., Skretas, G., Strauch, E.-M., Chari, N.S. and G. Georgiou, " Efficient Production of Membrane- Integrated and Detergent-Soluble G Protein-Coupled Receptors in Escherichia coli”  Protein Sci pdf  
• Faulkner MJ, Veeravalli K, Gon S, Georgiou G, Beckwith J, "Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways," Proc Natl Acad Sci 105(18):6735-6740 (2008). pdf
• Li, H., Hwang, B-Y., Lamikanthan, G., Blaber, S.I., Blaber, M., Golubkov, P. A., Ren, P., Iverson, B.L. and G. Georgiou, “The Substrate Specificity of Kallikrein 1 and 6 Determined by Phage Display,” Prot Sci  17(4):664-72 (2008). pdf
• Cobaugh, C.W., M.S.; Almagro, J.C., Pogson, M., Iverson, B.L. and G. Georgiou, “Synthetic Antibody Libraries Focused Towards Peptide Ligands,” J. Mol. Bio 378(3):622-33 (2008) PMCID: PMC2494707 pdf
• Varadarajan, N., Rodriguez S., Hwang, B-Y., Georgiou G. and B.L Iverson, “Highly active and selective endopeptidases with programmed substrate specificities,” Nature Chem. Biol. 4:290-4 (2008). PMID: 18391948 pdf
• News and Views Nature Chem. Bio 4:290-4 (2008)
• Selected as a “Recommended” paper www.facultyof1000.com
• Masip, L., Klein D., Quan S., Bardwell J.A.C. and G. Georgiou, “Laboratory Evolution of Escherichia coli thioredoxin for enhanced catalysis of protein oxidation in the periplasm reveals a phyolgentically conserved substrate specificity determinant” J. Bio. Chem. 283(2):840-8 (2008) PMID: 18003618 pdf

2007

• Strauch E.-M. and G. Georgiou, “E. coli tatC mutations that suppress defective twin-arginine transporter signal peptides,” J. Mol Bio. 374:283-91 (2007) PMCID: PMC2661573  pdf
• Link, J.A., Jeong, K-J. and G. Georgiou, “Beyond Toothpicks: New methods for isolating mutant bacteria ” Nature Rev. Microbiol. 5:680-8 (2007) PMID: 17676054 pdf
• Jeong, K-J., Seo M-J., Iverson, B.L. and G. Georgiou, “APEx 2-hybrid, a quantitative protein-protein interaction assay for antibody discovery and engineering” Proc. Natl. Acad.. Sci. U.S.A. 104;8247-52 (2007) PMCID: PMC1895937 pdf
• Jung, S.T., Jeong, K.J., Iverson, B.L. and Georgiou, G. "Binding and Enrichment of Escherichia coli Spheroplasts Expressing Inner Membrane Tethered scFv Antibodies on Surface Immobilized Antigens" Biotechnol & Bioeng. 98:39-47 (2007)PMID: 17657772 pdf
• Strauch E.-M and G. Georgiou, “A Bacterial Two-Hybrid System Based on the Twin Arginine Transporter Pathway of E.coli”  Protein Sci. 16:1001-8 (2007) PMCID: PMC2206650   pdf
• Ribnicky, B., Van Blarcom, T. and G. Georgiou, “A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding,” J. Mol. Bio 369:631-9 (2007) PMCID: PMC1995598 pdf
• Mazor, Y., Van Blarcom, T., Mabry, R., Iverson, B.L. and G. Georgiou, “Isolation of Engineered Full-Length Antibodies from Libraries Expressed in E. coli” Nature Biotechnology  25:563-5 (2007). PMID: 17435747pdf
• Nature 447:741-3 (2007);  News & Views in Nature Biotechnology 25:537-8 (2007); covered in numerous science news web sites
• Selected as a “Must Read” paper, www.facultyof1000.com
• Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP. "Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1," Proc Natl Acad Sci U.S.A. 1;104(18):7379-84 (2007). pdf
• Levy, R., Molineux, I., Iverson, B.L. and G. Georgiou, “Isolation of trans-Acting Genes that Enhance Soluble Expression of scFv Antibodies in the E.coli Cytoplasmby Lambda Phage Display,” J. Immunol. Meth.  10:164-173 (2007) PMID: 17328908 pdf
• Tullman-Ercek D, DeLisa MP, Kawarasaki Y, Iranpour P, Ribnicky B, Palmer T, Georgiou G. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides" J Biol Chem 282:8309-16 (2007) PMID: 17218314 pdf
• Hwang, B.Y., Varadarajan, N., Li, H., Rodriguez, S., Iverson, B.L. and Georgiou, G. "Substrate Specificity
  of the Escherichia coli Outer Membrane Protease OmpP" J Bacteriol. 189:522-30 (2007) PMCID: PMC1797397 pdf

2006

• Griswold, K.E., Aiyappan, N.S., Iverson, B.L. and G. Georgiou, "The Evolution of Catalytic Efficiency and Substrate Promiscuity in Human Theta Class 1-1 Glutathione Transferase"  J. Mol. Biol. 364:400-10 (2006) PMCID: PMC1995603 pdf
• Gao, J., Lee, K., Zhao, M., Qiu, J., Zhan, X., A. Saxena, Cohen, S.N. and G. Georgiou, “Differential Modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome, ” Mol  Microbiol 61:394-406 (2006) PMID: 16771842 pdf
• Selected as a “Recommended” paper, www.facultyof1000.com
• Lee, P.A., Tullman-Ercek, D. and G. Georgiou, “The Bacterial Twin Arginine Translocation Pathway” Ann Rev. Microbiol  60: 373-95 (2006) PMCID: PMC2654714 pdf
• Mabry, R.,  Brasky R., Geiger, R., Carrion Jr. R.,  Hubbard, G.B., Leppla, S., Patterson, J.L., Georgiou, G.,  Iverson, B.L. “Detection of Anthrax Toxin in the Circulation of Animals Infected with Bacillus anthracis by using Engineered Immunoassays,” Clinical and Vaccine Immunol 13:671-6 (2006) PMCID: PMC1489546 pdf        
• Masip, L., Veeravalli, K and G. Georgiou “The Many Faces of Glutathione,” Antiox. Redox Signal. 8:753-62 (2006 )PMID: 16771667 pdf
• Sweeney, R.Y., Park, E.Y., Iverson B.L. and G. Georgiou “Assembly of MultimericPhage Nanostructures through Leucine Zipper Interactions” Biotechnol. & Bioeng 20:539-45 (2006) PMID: 16897782 pdf
• Zhao, M., Kawarasaki, Y., Zhou L. and G. Georgiou, “Regulation of RraA, a Protein Inhibitor of RNase E-Mediated RNA Decay,” J. Bacteriol. 188:3257-63 (2006) PMCID: PMC1447450 pdf
• Pothukuchy, A., Mano, N., Georgiou, G. and A. Heller “A potentially insect-implantable trehalose electrooxidizing anode,” Biosensors & Bioelectron. 22:678-84 (2006) PMID: 16546370 pdf
• Harvey, B.R., Shanafelt, A.,  Baburina, I., Hui, R., Vitone, S., Iverson, B.L. and G. Georgiou, “Engineering of Recombinant Antibody  Fragments to Methamphetamine by Anchored Periplasmic Expression (APEx)” J. Immunol Methods 20:43-52 (2006) PMID: 16337958 pdf
• Segatori, L., Murphy, L., Arredondo, S., Kadokura, H., Beckwith, J., Gilbert, G. and G. Georgiou, “Conserved Role of the a-helix linker of the Bacterial Disulfide Isomerase DsbC in the Avoidance of Misoxidation by DsbB,” J. Biol. Chem.  281:4911-9 (2006)PMID: 16280324 pdf

2005

• Mabry, R., Rani, R., Geiger, R., Hubbard, R., Carrion, R., Brasky, K., Patterson, J., Georgiou, G. and B.L. Iverson “High Affinity Antibody Fragments Lacking Fc Regions Confer Protection Against Inhalation Anthrax,” Infect & Immun 73:8362-8 (2005). PMCID: PMC1307075
• Jeong, K.-J., Mabry, R. and G. Georgiou, “Avimers Hold their Own” Nature Biotechnol. 23:493-4 (2005). PMID: 16333289
• Pressman,  J.G., Georgiou G., Speitel G. E. Jr. “Scale-up Considerations for a Hollow-Fiber-Membrane Bioreactor Treating Trichloroethylene-Contaminated Water,” Water Environ Res. 77:533-42 (2005). PMID: 16274088
• Drummond, D.A., Iverson, B.L., Georgiou, G. and Arnold, F.H., "Why High-error-rate Random Mutagenesis Libraries are Enriched in Functional and Improved Proteins" J. Mol. Biol. 350:806-16 (2005).
• Griswold, K.E., Kawarasaki, Y., Ghoneim, N., Benkovic, S.J., Iverson, B.L., and G. Georgiou, "Evolution of highly active enzymes by homology-independent recombination" Proc. Natl. Acad.. Sci. U.S.A. 102:10082-87 (2005).
• Sheoran, A.S., Chapman-Bonofiglio, S., Harvey, B.R., Mukherjee, J., Georgiou, G., Donohue-Rolfe, D. and S. Tzipori,  “Humanantibody against Shiga toxin 2 administered to piglets after the onset of diarrhea due to  Escherichia coli O157:H7 prevents fatal systemic complications,”  Infect. & Immun. 73:4607-13.
  
• Varadarajan, N., Gam J. Olsen, M.A., Georgiou, G. And B.L. Iverson, ”Engineering of Protease Variants Exhibiting High Catalytic Activity and Exquisite Substrate Specificity”  Proc. Natl. Acad.. Sci. U.S.A. 102:6855-60 (2005).
• Hicks, M., Lee, P.A., Georgiou, G., Berks, B.C. and T. Palmer, "Positive Selection for Loss-of-Function tat Mutations Identifies Critical Residues Required for TatA Activity" J. Bacteriol. 187:2920-5 (2005).
• Goldman, E.R., Medintz, I.L., Hayhurst, A., Anderson, G.P., Mauro, M., Iverson, B.L., Georgiou, G. and Mattoussi, H. “Luminescent Quantum Dot Bioconjugates Prepared Using Proteins Engineered with Histidine Tags” Analytica Chimica Acta 534:63-7 (2005).
• Lin, S.C. and G. Georgiou, “A Biocatalyst for the Removal of Sulfite from Alcoholic Beverages” Biotech & Bioeng., 89:123-127 (2005).

2004

• Sweeney, R.Y., Mao, C., Gao, X., Burt, J.L., Belcher, A.M., Georgiou, G. and B.L. Iverson, “Bacterial Biosynthesis of Cadmium Sulfide Nanocrystals,” Chemistry & Biology 11:1553-9 (2004).  
• Jeong, K.J., Kawarasaki, Y., Gam, J.S., Harvey, B.R., Iverson, B.L. and Georgiou, G., “A Periplasmic Fluorescent Reporter Protein and its Application in High-throughput Membrane Protein Topology Analysis” J. Mol. Biol. 341:901-909 (2004). 
• Zhang, M., Monzingo, A.F., Segatori, L. , Georgiou, G. and J. D. Robertus, “The X-ray Structure of DsbC from Haemophilus influenzae” Acta Crystal. D 60:1512-8 (2004).
• McCarter, J.D., Stephens, D., Shoemaker, K., Rosenberg, S., Kirsch, J.F. and G. Georgiou, “Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpT” J. Bacteriol. 186:5919-25 (2004).
• Segatori, L. Gilbert H.G. and G. Georgiou, “Engineered DsbC Chimeras that Catalyze Both Protein Oxidation and Disulfide Bond Isomerization in E.coli: Reconciling Two Competing Pathways” Proc. Natl Acad. Sci. U.S.A. 101:10018-23 (2004). 
• Harvey, B., Georgiou G., Hayhurst, A., Jeong, K.J., Iverson, B.L. and G. Rogers, “Anchored Periplasmic Expression (APEx):   A Versatile Technology for the Isolation of High Affinity Antibodies from Combinatorial Libraries,”  Proc. Natl Acad. Sci. U.S.A. 101:9193-8 (2004). 
• Freeman, A., Cohen-Hadar, N., Abramov, S., Modai-Hod, R., Dror, Y and G. Georgiou,” Screening of a Million Cell Population by “Immobilized Cell on Immobilized Bead,” Approach,” Biotech & Bioeng 86:654-660 (2004).  
• Zhan X.,  Gao, J., Jain, C., Cieslewicz, M., Swartz, J.R. and  G. Georgiou, “Genetic Analysis of Disulfide Isomerization in E. coli:  The Expression of DsbC is Modulated by RNase E-Dependent mRNA  Processing,”    J.Bacteriol. 186:654-60 (2004).
• Masip, L., Pan, J., Haldar, S., Penner-Hahn, J.A., Delisa, M.P., Georgiou, G., Bardwell, J.C.A. and J.F. Collet, "Engineering a de novo Pathway for the Formation of Protein Disulfide Bonds in Bacteria," Science 303: 1185-1189 (2004).
• Mao, C., Solis, D.J., Reiss, B.D., Kottmann, S.T., Sweeney, R.Z., Hayhurst, A., Georgiou, G., Iverson, B.L. and A.M. Belcher,"Virus-Based Toolkit for the Directed Synthesis of Magnetic and Semiconducting Nanowires," Science 303: 213-17 (2004).
• DeLisa, M.P., Lee, P., Palmer, T. and G. Georgiou, ”Phage Shock Protein A (PspA) Relieves the Saturation of Protein Export Via the Tat Pathway,” J. Bacteriol. 186:366-73 (2004).

2003

• Kawarasaki, Y., Griswold, K.E., Selzer, T., Benkovic, S.J., Iverson, B.L. and G. Georgiou “Enhanced Crossover SCRATCHY: Construction and High-throughput Screening of a Combinatorial Library Containing Multiple Non-Homologous Crossovers,” Nucleic Acid Res. 31:E126 (2003).
• Mao, C., Flynn, C.E., Hayhurst, A., Sweeney, S., Qi, J., Georgiou, G., Iverson, B.L., and A. M. Belcher “Synthesis and Organization of Nanoscale II-VI Semiconductor Materials Using Evolved Peptide Specificity and Viral Capsid Assembly” J. Materials Chemistry 13:2414-21 (2003).
• Lee, K., Zhan X., Gao, J. Qiu, J., Feng, Y., Meganathan, R., Cohen, S.N. and G. Georgiou, “RraA: A Protein Inhibitor of RNase E activity that Globally Modulates RNA Abundance in E. coli,” Cell 114:623-644 (2003).
• Monzingo, A.F., Gao, J., Qiu, J., Zhan, M., Georgiou, G. and J.D. Robertus, J.D., “The X-ray Structure of E.coli RraA (MenG), a Protein Inhibitor of RNA Processing,” J. Mol. Biol. 332:1015-24 (2003).
• Goldman, E.R., Hayhurst, A., Lingerfelt, B.M., Iverson,B.L., Georgiou, G., and A.P. Anderson "2,4,6-Trinitrotoluene Detection Using Recombinant Antibodies," J. Environ. Monit. 5: 380-3 (2003).
• Georgiou, G. and L. Masip, "An Overoxidation Journey with a Return Ticket," Science 300: 592-594 (2003).
• Mao, C., Flynn, C.E., Hayhurst, A., Sweeney, S., Qiu, J., Georgiou, G., Iverson, B.L., and A.M. Belcher, "Viral Assembly of Oriented Quantum Dot Nanowires" Proc. Natl. Acad. Sci. U.S.A. 100: 6946-51 (2003).
• Delisa, M.P., Tullman, D. and G. Georgiou, "Folding Quality Control in the Export of Proteins via the Bacterial Twin Arginine Translocation Pathway" Proc. Natl. Acad. Sci. U.S.A. 100: 6115-29 (2003).
• Hayhurst, A., Happe, S., Mabry, R., Iverson, B.L. and G. Georgiou, "Isolation and Expression of Recombinant Antibody Fragments to the Biological Warfare Pathogen Brucella melitensis" J. Immunol. Methods 276: 185-196 (2003).
• Griswold, K., Mahmood, N., Iverson, B.L. and G. Georgiou, "Effects of Codon Usage on the Expression of Fusarium solani Cutinase in the Escherichia coli Cytoplasm" Prot. Express. Purification 27:134-142 (2003).

2002

• Georgiou, G. "Minireview: How to Flip a (Redox) Switch" Cell 111: 607-610 (2002).
• Cox, J.C., Hayhurst, A., Hesselberth, J., Bayer, T.S., Georgiou, G. and Ellington, A.D. "Automated Selection of Aptamers Against Protein Targets Translated in vitro: From Gene to Aptamer" Nucleic Acid Res. 30: 108-122 (2002).
• Chen, W. and G. Georgiou, “Cell Surface-Display of Heterologous Proteins: From High Throughput Screening to Environmental Applications,” Biotechnol. & Bioeng. 79:496-503 (2002).
• Delisa, M., Samuelson, P., Palmer, T. and G. Georgiou, "Genetic Analysis of the Twin Arginine Translocator Secretion Pathway in Bacteria”, J. Biol. Chem. 277:29825-31 (2002).
• Maynard, J.A., Leppla, S.H., Bratt, K., Patterson, J.L., Iverson, B.L. and G. Georgiou, “Antibody Mediated Protection to Anthrax Toxin Challenge Correlates with Affinity”, Nature Biotechnology 20:577-601 (2002).
• Maynard, J., Chen, G., G. Georgiou and B. L. Iverson, “In vitro Scanning Saturation Mutagenesis,” Methods in Mol Biol 182:149-63 (2002).

2001

• Hayhurst, A. and G. Georgiou, “High Throughput Antibody Isolation”, Curr. Opin. Chemical Biology 5:683-689 (2001).
• Chen G., Hayhurst, A. Thomas, J.G., Harvey, B.R., Iverson B.L. and G. Georgiou, “Isolation of High Affinity Ligand-Binding Proteins by Periplasmic Expression with Cytometric Screening (PECS)” Nature Biotechnol. 19:537-42 (2001).
• Levy, R., Weiss, R., Chen, G., Iverson, B.L. and G. Georgiou, “Production of Correctly Folded Fab Antibody Fragment in the Cytoplasm of Escherichia coli trxB gor Mutants via the Coexpression of Molecular Chaperones” Prot. Express. Purification 23 (2):338-347 (2001).
• Liu, C. Y., Georgiou, G. and G.E. Speitel Jr., “Kinetics of MTBE Co-Metabolism by Propane and Butane Degrading Bacteria,” Appl. Environ. Microbiol. 67:2197-2201 (2001).
• Bessette, P.H., Qiu, J., Bardwell, J.C.A., Swartz, J.R. and G. Georgiou, “Effects of Sequences of the Active Site Dipeptides of DsbA and DsbC on In Vivo Folding of Multidisulfide Proteins in Escherichia coli,” J. Bacteriol 183:980-88 (2001).

2000

• Yarnitzky, C., Campbell, C.N., Caruana, A.-M., Georgiou, G. and A. Heller “Potentiodynamic On-Line Monitoring of the Concentration of Glucose in Bioreactors,” J. Electroanal. Chemistry 491:154-159 (2000).
• Razatos, A., Ong, Y.L., Boulay, F., Elbert, D.L., Hubbell, J.A., Sharma, M.M. and G. Georgiou, “Force Measurements Between Bacteria and Poly(ethylene glycol)-Coated Surfaces,” Langmuir 16:9155-9158 (2000).
• Georgiou, G. “The Analysis of Large Protein Libraries Using Flow Cytometry” Adv. Protein. Sci. 55:297-315 (2000).
• Maynard, J. A. and G. Georgiou, “Antibody Engineering,” Annual Rev. Biomedical Engineering 2: 339-76 (2000).
• Olsen, M., Stephens, D.L., Griffiths, D., Daugherty, P.S., Georgiou, G. and B.L. Iverson, “Function-Based Isolation of Novel Enzymes from a Large Library” Nature Biotechnology 18:1071-76 (2000).
• Daugherty, P.S., Iverson, B.L. and G. Georgiou, “Flow Cytometric Screening of Cell-based Libraries” J. Immunol. Methods 243:2110227 (2000).
• Olsen, M., Iverson, B.L. and G. Georgiou, “High Throughput Screening of Enzyme Libraries,” Curr. Opin. Biotechnol. 11:331-337 (2000).
• Pressman, J.G., Georgiou, G., and G.E. Speitel, Jr. “Membrane Bioreactor for the Removal of Trichloroethyelene from the Vapor Phase” Biotechnol. & Bioeng. 68:548-556 (2000).
• Daugherty, P.S., Chen, G., Iverson, B.L. and G. Georgiou, “Quantitative Analysis of the Effect of the Mutation Frequency on the Affinity Maturation of single chain Fv Antibodies,” Proc. Natl. Acad. Sci. U.S.A. 97:2029-34 (2000).
• Razatos, A. and G. Georgiou, "Evaluating Bacterial Adhesion Using Atomic Force Microscopy," In Handbook of Bacterial Adhesion, Y.H. An and R.T. Friedman (Eds), Humana Press (2000).

1999

• Georgiou, G. and N. Dewitt, “Enzyme Beauty,” Nature Biotechnol. 17:1161-1162 (1999).
• Bessette, P.H., Åslund, F., Beckwith, J. and G. Georgiou “Efficient Folding of Proteins with Multiple Disulfide Bonds in the Escherichia coli Cytoplasm,” Proc. Natl. Acad. Sci. U.S.A. 96:13703-8 (1999).
• Georgiou, G. and P. Valax, "Isolation of Inclusion Bodies from Bacteria" Methods Enzymol. 309:48-58 (1999).
• Aziz, C.E., Georgiou, G. and G.E. Speitel, Jr. "Cometabolism of Binary Chlorinated Solvent Mixtures Using Methylosinus trichosporium OB3b PP358," Biotechnol. & Bioeng. 65:100-107 (1999).
• Zhan, M., Schwaller, M., Gilbert, H.G. and G. Georgiou, "Facilitating the Formation of Disulfide Bonds in the E. coli Periplasm via Co-Expression of Yeast Protein Disulfide Isomerase" Biotechnol. Prog. 15:1033-38 (1999).
• Hoskins, B.C., Fevang, L., Majors, P.D., Sharma, M.M. and G. Georgiou, "Selective Imaging of Biofilm Growth by NMR Relaxation," J Magn Reson 139(1): 67-73 (1999).
• Daugherty, P.S., Olsen, M., Iverson, B.L. and G. Georgiou, "Development of an Optimized Expression System for the Screening of Antibody Libraries Displayed on the Escherichia coli Surface" Protein Eng 12(7): 613-621 (1999).
• Chen, G., Dubrawsky, I., Martinez, P., Georgiou, G. and B.L. Iverson, "In vitro Scanning Saturation Mutagenesis of all the Specificity Determining Residues in an Antibody Binding Site," Protein Eng. 12: 349-356 (1999).
• Ong, Y., Razatos, A., Georgiou, G. and M.M. Sharma, "Adhesion Behavior of Bacteria with Biomaterials Surfaces" Langmuir 15: 2719-2725 (1999).
• Park, S.J., Georgiou, G. and S.Y. Lee, "Sectretory Production of Recombinant Protein by a High Cell Density Culture of a Protease negative Mutant of Escherichia coli," Biotechnol. Progr. 15: 164-168 (1999).
• Bessette, P., Cotto, J.J., Gilbert, H. G. and G. Georgiou, "In vivo and in vitro Function of the E. coli Periplasmic Cysteine Oxidoreductase DsbG," J. Biol. Chem. 274(12): 7784-7792 (1999).
• De Lumley-Woodyear, T., Campbell, C.N., Freeman, E., Freeman, A., Georgiou, G. and A. Heller, "Rapid amperometric verification of PCR amplification of DNA" Anal. Chemistry 71: 535-538 (1999).
• Pressman, J.G., Georgiou, G. and G.E. Speitel, Jr. "Demonstration of Efficient Trichloro- ethylene Degradation in a Hollow Fiber Membrane Bioreactor" Biotechnol. & Bioeng. 62: 681-93 (1999).
• Freeman, A., Abramov, S. and G. Georgiou, "Site-Protected Fixation and Immobilization of Escherichia coli Cells Displaying Surface Anchored Beta-lactamase," Biotechnol. & Bioeng. 62:155-160 (1999).

1998

• Qiu, J., Swartz, J.R. and G. Georgiou, "Expression of Active, Full Length Human Tissue Plasminogen Activator in Escherichia coli," Appl. Environ. Microbiol. 64:4891-4896 (1998).
• Razatos, A., Ong, Y.L., Sharma, M.M., and G. Georgiou, "Use of Atomic Force Microscopy to Investigate the Adhesion of Bacteria onto Biomaterials," J. Biomaterials Sci. Polymer Ed. 9:1361-73 (1998).
• Daugherty, P.S., Chen, G., Olsen, M.J., Iverson, B.L. and G. Georgiou, "Rapid and Quantitative Screening of Antibody Libraries Using Bacterial Cell Surface Display and Fluorescence-Activated Cell Sorting" Protein Eng. 11:825-92 (1998).
• Razatos, A., Ong, Y., Sharma, M.M. and G. Georgiou, "Molecular Determinants of Bacterial Adesion Analyzed by Atomic Force Microscopy," Proc. Natl. Acad. Sci. U.S.A. 95:11059-11064 (1998).
• Stumpe, S., Uhlenmann, E., Stephens, D.L., Georgiou, G. and E. P. Bakker, "Identification of OmpT as the Protease that Hydrolyzes the Antimicrobial Peptide Protamine Before it Enters Growing Cells of Escherichia coli," J. Bacteriol. 180:4002-4006 (1998).

1997

• Rietsch, A., Bessette, P., Georgiou, G. and J. Beckwith, "Reduction of the Periplasmic Disulfide Bond Isomerase DsbC, Occurs by Passage of Electrons from Cytoplasmic Thioredoxin" J. Bacteriol. 179:6602-6608 (1997).
• Georgiou, G., Stathopoulos, C., Daugherty, P., Nayak, A.R., Iverson, B.L. and R. Curtiss, III, "Display of Heterologous Molecules on the Surface of Microorganisms: From the Screening of Combinatorial Libraries to Live Bacterial Vaccines," Nature Biotechnol. 15:29-35 (1997).
• Burks, E.A., Chen, G., Georgiou, G. and B.L. Iverson, "In vitro Scanning Saturation Mutagenesis of an Antibody Binding Pocket," Proc. Natl. Acad. Sci. U.S.A. 94:412-7 (1997).

1996

• Fitch, Mark W., Weissman, D., Phelps, P., Georgiou, G., and G.E. Speitel Jr., "Trichloroethylene Degradation by Methylosinus trichosporium OB3b in a Sequencing Biofilm Reactor" Water Research 30:2655-2664 (1996).
• Chen, G., Cloud, J., G. Georgiou and B.L. Iverson, "A Quantitative Immunoassay Utilizing Escherichia coli Cells Possessing Surface-Exposed Single Chain Fv Molecules," Biotech. Prog. 12:572-74 (1996).
• Freeman, A., Abramov, S. and G. Georgiou, "Fixation and Stabilization of E.coli Cells Displaying Genetically Engineered Cell Surface Proteins," Biotechnol. & Bioeng. 52:625- 630 (1996).
• Ostermeier, M., DeSutter K. and G. Georgiou "The Eucaryotic Protein Disulfide Isomerase Complements Escherichia coli dsbA Mutants and Increases the Yield of Heterologous Secreted Proteins with Disulfide Bonds," J. Biol. Chem. 271:10616-10622 (1996).
• Georgiou, G. and P. Valax, "Expression of Correctly Folded Proteins in Escherichia coli," Curr. Opin. Biotechnol. 7: 190-197 (1996).
• Fitch, M. W., Speitel Jr., G. E., and G. Georgiou, "Degradation of Trichloroethylene by Methanol-Grown Cultures of Methylosinus trichosporium OB3b," Appl. Environ. Microbiol. 62:1124-1128 (1996).
• Georgiou, G., Stephens, D.,L. Stathopoulos C., Poetschke, H.L., Mendenhall, J. and C.F. Earhart., "Display of b-lactamase on the Escherichia coli Surface: Outer Membrane Phenotypes Conferred by Lpp'-OmpA'-ß-lactamase Fusions," Protein Eng. 9:239-247 (1996).
• Stathopoulos, C., Georgiou, G. and C. F. Earhart, "Characterization of Escherichia coli expressing an Lpp'OmpA(46-159)-PhoA fusion protein localized in the outer membrane." Appl. Microbiol. Biotechnol 45:112-119 (1996).

1995

• Aziz, C.E., Fitch, M.W., Linquist, L., Pressman, J., Georgiou, G. and G.E. Speitel Jr., "Degradation of Trichloroethylene by Methylosinus trichosporium OB3b PP358 in a Hollow Fiber Membrane Bioreactor," Env. Sci. Technol. 29:2574-2583 (1995).
• Georgiou, G., "Expression of Proteins in Bacteria," in Principles and Applications of Protein Engineering, J.L. Cleland and C.S. Craik (Eds.) (1995).

1994

• Meerman, H. and G. Georgiou, "Construction and Characterization of a Set of E.coli Strains Deficient in All Known Loci Affecting the Proteolytic Stability of Secreted Recombinant Proteins," Bio/Technology 12:1107-1110 (1994).
• Georgiou, G., Valax, P., Ostermeier, M. and P. Horowitz, "Folding and Aggregation of TEM ß-lactamase: Analogies with the Formation of Inclusion Bodies in Escherichia coli," Protein Sci. 3:1953-1960 (1994).
• Ostermeier, M. and G. Georgiou, "The Folding of Bovine Pancreatic Trypsin Inhibitor in E.coli periplasm," J. Biol. Chem. 269:21072-21077 (1994).
• Francisco, J.A. and G. Georgiou, "The Expression of Recombinant Proteins on the External Surface of Escherichia coli: Biotechnological Applications," Biochemical Eng VIII, Ann. NY Acad. Sci. 745:372-382 (1994).
• Sarkar, A.K., Georgiou, G. and M.M. Sharma, "Transport of Bacteria in Porous Media: Part II," Biotechnol. & Bioeng. 11:499-508 (1994).
• Sarkar, A.K., Georgiou, G. and M.M. Sharma, "Transport of Bacteria in Porous Media. Part I: An Experimental Investigation," Biotechnol. & Bioeng. 11:489-497 (1994).
• Lin, S.C., Carswell, K.F., Sharma, M.M. and G. Georgiou, "Continuous Production of the Lipopetide Biosurfactant from Bacillus licheniformis JF-2," Appl. Microbiol. Biotechnol. 44:281-285 (1994).
• Meerman, H. J. and G. Georgiou, "High Level Production of Proteolytically Sensitive Secreted Proteins in E.coli Strains Impaired in the Heat Shock Response," Recombinant DNA III, Ann. NY Acad. Sci. 721:292-302 (1994).
• Przybycien, T.M., Dunn, J.P., Valax, P. and G. Georgiou, "Secondary Structure Characterization of ß-lactamase Inclusion Bodies," Protein Eng. 7:131-136 (1994).
• Lin, S.C., Minton, M.A., Sharma, M.M. and G. Georgiou, "Purification, Structure and Immunological Characterization of a Biosurfactant Produced by Bacillus licheniformis JF-2," Appl. Environ. Microbiol. 60:31-38 (1994).

1993

• Francisco, J.A., Campbell, R., Iverson, B.L. and G. Georgiou, "Production and Flow Cytometry Sorting of Escherichia coli Expressing a Single Chain Fv Antibody on its Surface," Proc. Natl. Acad. Sci. U.S.A. 90:10444-10448 (1993).
• Valax, P. and Georgiou, G., "Molecular Characterization of Protein Inclusion Bodies in Escherichia coli: I. Composition," Biotechnology Progress 9:539-547 (1993).
• Fitch, M.W., D. Graham, Arnold, R., Speitel, G.E. Jr. and G. Georgiou, "Phenotypic Analysis of Copper Resistant Mutants of Methylosinus trichosporium OB3b," Appl. Environ. Microbiol. 59:2771-2776 (1993).
• Francisco, J.A., Stathopoulos, C., Warren, R.A.J., Kilburn, D.G. and G. Georgiou, "Specific Adhesion and Hydrolysis of Cellulose by intact Escherichia coli Cells Expressing Surface Anchored Cellulase or Cellulose Binding Domain," Bio/Technology 11:491-496 (1993).
• Lin, S.-C., Sharma, M.M. and G. Georgiou, "Production and Deactivation of the Lipopeptide Surfactant from Bacillus licheniformis JF-2," Biotechnol. Progress 9:138-145 (1993).
• Georgiou, G., Poetschke, H.L., Stathopoulos, C. and J.A. Francisco, "Engineering of Bacterial Surfaces for Biotechnology Applications," Trends in Biotechnol. 11:6-10 (1993).

1992

• Thomas, D. A. Carswell, K. and G. Georgiou, "Degradation of Chlorinated Biphenyls by the White Rot Fungus Phanerochaete chrysosporium," Biotechnol. Bioeng. 40:1395-1402 (1992).
• Phelps, P., Agrawal, S., Speitel, G. E. Jr. and G. Georgiou, "Copper-Resistant Mutants of Methylosinus Trichosporium Ob3b Suitable for the Degradation of Trichloroethylene," Appl. Environ. Microbiol. 58:3701-3708 (1992).
• Francisco, J.A., Earhart, C.F. and Georgiou, G. "Transport and Anchoring of ß-lactamase to the External Surface of Escherichia coli," Proc. Natl. Acad. Sci. U.S.A. 89:2713-2718 (1992).
• Valax, P. and G. Georgiou, "Characterization and Refolding of ß-lactamase Inclusion Bodies from Escherichia coli," in Biocatalyst Design for Stability and Specificity, M.E. Himmell and G. Georgiou (Eds.), ACS Symposium Series 516:126-139 (1992).
• Bowden, G.A., Baneyx, F. and G. Georgiou, "Abnormal Fractionation of ß-lactamase in Escherichia coli: Evidence for an Interaction with the Cytoplasmic Membrane in the Absence of a Leader Peptide," J. Bacteriology 174: 3407-10 (1992).
• Baneyx, F. and G. Georgiou, "The Degradation of Secreted Proteins in Escherichia coli," Ann. N.Y. Acad. Sci. 665:301-308 (1992).
• Baneyx, F. and G. Georgiou, "The Degradation of Recombinant Proteins in Bacteria," in Pharmaceutical Biotechnology v. 2, T.J. Ahern and M. Manning (Eds.), Plenum Press (Publ.), (1992).
• Georgiou, G., Lin, S.C. and M.M. Sharma, "Recent Developments in Microbial Surfactants," Bio/Technology 10:60-65 (1992).

1991

• Baneyx, F., Thomas, D., Ayling, A., Palumbo, T, and G. Georgiou, "Optimization of Growth Conditions for the Production of Proteolytically Sensitive Proteins in the Periplasmic Space of Escherichia coli," Appl. Microbiol. Biotechnol. 36:14-20 (1991).
• Bowden, G., Paredes, A. and G. Georgiou, "Structure and Morphology of Protein Inclusion Bodies in Escherichia coli," Bio/Technology 9:725-730 (1991).
• Baneyx, F. and G. Georgiou, "Construction and Characterization of Escherichia coli Strains Deficient in Multiple Secreted Proteases: Protease III Degrades High Molecular Weight Substrates In-vivo," J. Bacteriology 173: 2163-71 (1991).
• DeBernardez-Clark, E.and G. Georgiou, "The Significance of Protein Folding in Biotechnology," in Protein Refolding, E. DeBernardez-Clark and G. Georgiou (Eds.), ACS Symposium Series 470:1-19 (1991).
• Valax, P. and G. Georgiou, "The Aggregation of ß-lactamase in-vitro," in Protein Refolding, G. Georgiou and E. DeBernardez-Clark (Eds.), ACS Symposium Series 470:97-101 (1991).

1990

• Bowden, G. and G. Georgiou, "Aggregation and Folding of ß-lactamase in Escherichia coli," J. Biol. Chem., 265:16760-6 (1990).
• Georgiou, G. and G. Bowden, "Inclusion Body Formation and the Recovery of Aggregated Recombinant Proteins," in Recombinant DNA Technology and Applications, C.S. Ho, A. Prokop & R. Bajpai (Eds.), McGraw-Hill (1990).
• Georgiou, G. and F. Baneyx, "Expression and Immobilization of a Protein A-ß-Lactamase Hybrid Protein," Ann. N.Y. Acad. Sci. 589:139-147 (1990).
• Baneyx, F., Schmidt, C. and G. Georgiou, "Affinity Immobilization of a Genetically Engineered Bifunctional Protein," Enzym. Microbial Technol. 12:337-42 (1990).
• Baneyx, F. and G. Georgiou, "In vivo Degradation of Secreted Fusion Proteins by the Escherichia coli Outer Membrane Protease OmpT," J. Bacteriology 172: 491-494 (1990).

1989

• Sarkar, A.K., Goursaud, J.C., Sharma, M.M. and G. Georgiou, "A Critical Evaluation of MEOR Processes," In situ 13:207-238 (1989).
• Georgiou, G. and J.A. Hubbell, "Protein Engineering" in Encyclopedia of Chemical Processing and Design, J.J. McKetta (Ed.), Marcel Dekker (Publ.) (1989).
• Georgiou, G., "Thermodynamics of Protein Stability," in Chemical Engineering Problems in Biotechnology, M.L. Shuler (Ed.), American Institute of Chemical Engineers (Publ.) (1989).
• Baneyx, F. and G. Georgiou, "Expression, Purification and Characterization of a Protein A-ß-lactamase Hybrid Protein," Enzym. Microbial Technol. 11:559-567 (1989).

1988

• Georgiou, G., "The Overproduction of Recombinant Proteins in Bacteria," AIChE J. 34:1233-48 (1988).
• Georgiou, G., Palumbo, T.A., Wilson, D.B. and M.L. Shuler, "The Effect of Alkaline Growth Medium on the Production of Secreted Proteins in Escherichia coli," Biotechnol. Letters 10:377-383 (1988).
• Bowden G. and G. Georgiou, "Effects of Sugars on ß-lactamase Aggregation in Escherichia coli," Biotechnol. Progress 3:97-101 (1988).
• Georgiou, G., Wilson, D.B. and M.L. Shuler, "Release of Periplasmic Enzymes and Other Physiological Effects of ß-lactamase Overproduction in Escherichia coli," Biotechnol. Bioeng. 32:742-748 (1988).

1986

• Georgiou G. and M.L. Shuler, "A Computer Model for the Growth and Differentiation of a Fungal Organism on Solid Substrates," Biotechnol. Bioeng. 28:405-417 (1986).
• Georgiou, G., Telford, J.N., Shuler M.L. and D.B. Wilson, "Localization of Inclusion Bodies in Escherichia coli Overproducing ß-lactamase or Alkaline Phosphatase," Appl. Environ. Microbiol. 52:1157-1161 (1986).

1985

• Georgiou, G., Chalmers, J.J., Shuler, M.L. and D.B. Wilson, "Continuous Production of Recombinant Proteins from Immobilized Recombinant E.coli Capable of Selective Protein Excretion," Biotechnology Progress 1:75-79 (1985).