Publications

2012-2014 2010-2011 Prior to 2010  

 

2014

Romain G., Senyukov, R.R., Merouane, A., Kelton, W., Liadi, M. A., Georgiou, G., Roysam, B., Lee, D.A. and N. Varadarajan, “Antibody Fc-engineering Improves Frequency and Promotes Kinetic Boosting of Serial Killing Mediated by Natural Killer Cells,” Blood (2014) pii: blood-2014-04-569061. [Epub ahead of print] PMID: 25232058.

DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine (accepted)

Lavinder, J.J., Hoi, K.H., Reddy, S.T., Wine, Y. and G. Georgiou, “Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire”, PLoS One 9:e101322 (2014) PMID: 24684310.

Boutz, D.R., Horton, A.P., Wine, Y., Lavinder, J., Georgiou, G. and E.M. Marcotte,” “Proteoimic Identification of Monoclonal Antibodies from Serum, Anal. Chem. (2014) PMID: 24684310.

Mroczek, E.S., Ippolito, G.C., Rogosch, T., Hoi, K.H., Hwangpo, T.A., Brand, M.G., Zhuang, Y., Liu, C.R., Schneider, D.A., Zemlin, M., Brown, E.E., Georgiou, G., Schroeder, HW Jr. “Differences in the Composition of the Human Antibody Repertoire by B Cell Subsets in the Blood,” Front.  Immunol. 19:96 (2014) PMID: 24678310.

Laserson, U., Vigneault, F., Gadala-Maria, D., Yaari, G., Uduman, M., Kasvin-Felton, J., Kelton, W., Jung, S.-T., Liu, Y.-P., Lasserson, J., Chari, R., Lee, J., Bachelet, I., Hickey, B., Lieberman-Aiden, E., Hanczaruk, B., Simen, B.B., Egholm, M., Koller, D., Georgiou, G., Kleinstein, S., George Church,  “High-Resolution Antibody Dynamics of Vaccine-Induced Immune Responses” Proc. Natl. Acad. Sci. USA 111:4928-4933(2014) PMID: 24639495.

Doria-Rose, N.A., Schramm, C.A., Gorman, J., Moore, P.L., Bhiman, J.N., DeKosky, B., Ernandes, M.J., Georgiev, I.S., Kim, H.J., Pancera, M., Staupe, R.P., Altae-Tran, H.R., Bailer, R.T., Crooks, E.T., Cupo, A., Druz, A., Garrett, J.N., Hoi, K.H., Kong, R., Louder, M.K., Longo, N.S., McKee, K., Nonyane, M., O'Dell, S., Roark, R.S., Rudicell, R.S., Schmidt, S.D., Sheward, D.J., Soto, C., Wibmer, C.K., Yang, Y. Zhang, Z., Mullikin, J.C., Binley, J.M., Sanders, R.W., Wilson, I.A., Moore, J.P., Ward, A.B., Georgiou, G., Williamson, C., Abdool Karim, S.S., Morris, L., Kwong, P.D., Shapiro, L., Mascola, J.R., “Developmental Pathway for Potent V1V2-Directed HIV-1-Neutralizing Antibodies,”  Nature 509:55-62 (2014)PMID: 24590074

 Commentaries: MedicalXpress: http://medicalxpress.com/news/2014-03-antibody-evolution-hiv-vaccine.html; Science Newsline: http://www.sciencenewsline.com/articles/2014030414220027.html;  Medical Nees Today: http://www.medicalnewstoday.com/releases/273504.php;
 Selected as a “Recommended” paper: http://f1000.com/prime/718297894

Georgiou G., Ippolito, G., Beuasang, G., Busse, C.E., Wardemann, H. and S.R. Quake, “The Promise and Challenge of High-throughput Sequencing of the Antibody Repertoire,” Nature Biotechnol. 32:158-68 (2014)
PMID: 24441474. 

Quandt, E.M., Deatherage, D.E., Ellington, A.D., Georgiou, G. and J. E. Barrick, “Recursive genome-wide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in E. coli,”  Proc. Natl. Acad. Sci. USA 111:2217-22 (2014) PMID: 24379390

      Commentaries: Phy.org: http://phys.org/news/2014-01-key-evolutionary-coli-metabolism.html;
National Geographic: http://phenomena.nationalgeographic.com/2014/01/06/evolution-hidden-in-plain-sight/; Proc. Natl. Acad. Sci. USA Highlight:.http://www.pnas.org/site/highlights/highlights.xhtml#trait; Proc. Natl. Acad. Sci. USA Commentary: http://www.pnas.org/content/111/6/2056.full; Le Scienze:
http://www.lescienze.it/news/2014/01/03/news/nuovo_tratto_successo_evolutivo_raffinamento-1946457/;  Technology.org: http://www.technology.org/2014/01/29/unpacking-past-identifying-key-evolutionary-step-e-coli-metabolism/

Lavinder, J., Wine, Y., Giesecke, C., Ippolito, G.C., Horton, A.P., Hoi, K.-H., DeKosky, B.J., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R. and G. Georgiou,  “Dynamics, Functionality and Discordance of the Human Serum Antibody and BCR Repertoires Following Immunization” Proc. Natl. Acad. Sci. USA 111:2259-64(2014) PMID: 24469811
                         Commentaries: Highlight, Proc. Natl. Acad. Sci: http://www.pnas.org/content/111/6/2049.full

2013

  • Tanios, R., Bekdash, A., Kassab. E., Stone, E., Georgiou, G., Frankel, A.E., Abi-Habib, R.J. “Human Recombinant Arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-Induced Arginine Depletion is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells,” Leuk Res. 37:1565-71 (2013) PMID: 24018014.
  • Paley, O., Agnello, G., Cantor, J., Yoo, T.H., Georgiou, G., Stone E.M., “GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria,” Methods Mol. Biol. 978:31-44 (2013) PMID: 23423887
  • DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Rawlings, M.P., Varadarajan, N., Wine, Y., Giesecke, C., Dörner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Wilson, C.P., Ellington, A.D. and G. Georgiou, “High Throughput Determination of the Antibody VH:VL Paired Repertoires,” Nature Biotechnol. 31:166-69(2013) PMID: 23334449
  • Needham, B.D., Carroll, S., Giles, D.K., Georgiou, G., Whiteley, M., and Trent, M.S.  “Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin” Proc. Natl. Acad. Sci. USA 110:1464-69 (2013) PMID: 23297218
     Commentaries Science: http://stm.sciencemag.org/content/5/170/170ec20.full; The Scientist:
    http://www.the-scientist.com//?articles.view/articleNo/34313/title/Bacteria-Boost-Vaccine-Effectiveness/
     Selected as a “Recommended” paper: http://f1000.com/prime/717982098#recommendations
  • Wine, Y., Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M., Ellington, A.D., Marcotte, E.M. and G. Georgiou,  “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA 110: 2993-8(2013) PMID: 23382245
  • Carroll, S. and G. Georgiou,“Antibody-Mediated Inhibition of Human C1s and the Classical Complement Pathway, “ Immunobiology doi:pii: S0171-2985(13)00006-5. 10.1016/j.imbio.2013.01.001 (2013). 
  • Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “Structural Ensemble of an Intrinsically Disordered Polypeptide” J Phys Chem B. 117:118-24 (2013) PMID: 23205890
  • Jung, S. T., Kelton, W., Kang, T. H.; Ng, D., Anderssen, J. T.; Sandlie, I., Sarkar, C., G. Georgiou,
    "Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity" ACS Chem. Bio. 8:368-75 (2013) PMID: 23030766

2012

  • Saggy, I., Wine, Y., Georgiou G. and Benhar, I., "Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining" PEDS (2012) PMID: 22988130
  • Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, "De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy," ACS Chem. Bio. (2012) PMID: 22963240
    Commentary in Chem & Eng News
  • Romero, P.A., Stone, E.M., Lamb, C., Chantranupong, L., Krause, A., Miklos, A.E., Hughes, R.A., Fechtel, B., Ellington, D.A., Arnold, F.H. and G. Georgiou, "SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis", ACS Synth. Biol. 1:221-28 (2012) PMID: 22737599
  • Li W., Cantor, J., Yogesha, S.D.,  Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M.,  and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation" ACS Chem. Bio. Epub ahead of print (2012) PMID: 22891768
  • Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation
    in the Structure of Human IgG Fc"  ACS Chem. Bio (2012) PMID: 22747430
  • Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T.,  Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV”  J. Virology 86:9113-21 (2012)  PMID: 2269665
  • Skretas, G.  Makino, T. Varadarajan N.,  Pogson M. and G. Georgiou,  “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coliMetabolic Eng. 14:591-602 (2012) PMID: 22609824 
  • Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E., Transl. Oncology 5:26-31 (2012) PMID: 22348173
  • Ippolito, G.C.,  Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
    and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One 7:e35497 (2012) PMID: 2255816
  • Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
    P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington,  “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,”  Chem. & Biol.  19:449-55 (2012) PMID: 22520751
  • Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou,  "Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2" ChemBiochem 3:649-53(2012) PMID: 22334509
  • Cantor,  J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno--genicity Enzymes for Amino Acid Depletion Therapy in Cancer,”  Methods Enzymol. 502:291-312 (2012). PMID:22208990
  • Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P., Chanhee, J. Stone, E.M., Georgiou, G. and Arthur E. Frankel, " Cytotoxicity of Human Recombinant Arginase I [Co] PEG5000 in the Presence of L-Citruline is Dependent on Arginosuccinate Synthetase Expression in Human Cells"Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999
  • Stone, E. M., Chantrranupong L., Gonzalez, C., O’Neal, J., Rani, M., VanDenBerg, C. and G. Georgiou, "Strategies for Optimizing the Serum Persistence of Engineered Human Arginase I for Cancer Therapy," J. Control. Release 58L171-9 (2012) PMID 22001609.