Publications

2009

Zhou, L., Zhao, M., Wolf, R.Z., Graham, D.E. and George Georgiou, "Transcriptional Regulation of the Escherichia coli rraB, Encoding a Protein Inhibitor of RNase E," Journal of Bacteriology (Accepted).

Pogson, M., Georgiou, G. and B.L. Iverson, “Engineering next generation proteases,” Curr Opin Biotechnol. 2009 (Epub ahead of print) PMID: 19709875

Arredondo SA, Chen TF, Riggs AF, Gilbert HF, Georgiou G. "The role of dimerization on the catalytic properties of the Escherichia coli disulfide isomerase DsbC," J Biol Chem. (2009 Jul 6) Epub ahead of print PMID: 19581640

Mazor Y, Van Blarcom T, Iverson BL, Georgiou G. “Isolation of full-length IgG antibodies from combinatorial libraries expressed in Escherichia coli,” Methods Mol Biol. 525:217-39, xiv (2009) PMID: 19252859

Varadarajan, N., Cantor, J., Georgiou, G. and B.L. Iverson, “Construction and flow cytometric screening of targeted enzyme libraries” Nature Protocol 4:893-901 (2009) PMID: 19478805 pdf

Leysath, C.E., Monzingo, A.F., Maynard, J.A., Barnett, J., Georgiou, G., Iverson, B.L. and Robertus, J.D. “Crystal Structure of the Engineered Neutralizing Antibody M18 Complexed to the Anthrax Protective Antigen”J Mol Biol. 3:387(3):680-93 (2009) PMCID: PMC2670345 pdf

Eser, M.,  Masip, L.,  Kadokura, H. Georgiou, G. and J.Beckwith, “Disulfide bond formation by exported glutaredoxin indicates glutathione's presence in the E. coli periplasm.,” Proc Natl Acad Sci U S A 106(5):1571-7 (2009) PMCID: PMC2635786 pdf

Seo, M.-J., Jeong K-J., Leysath, C.E., Ellington, A.D., Iverson, B.L. and G. Georgiou, “Engineering Antibody Fragments to Fold in the Absence of Disulfide Bonds,”Prot Sci. 18:259-67 (2009). PMCID: PMC2635786 pdf

Skretas G. and G. Georgiou, “Genetic Analysis of G Protein-Coupled Receptor Expression in Escherichia coli: Inhibitory Role of DnaJ on the Membrane Integration of the Human Central Cannabinoid Receptor,”  Biotechnol Bioeng. 1;102(2):357-67(2009) PMCID: PMC2666920 pdf

2008

Mazor, Y., Van Blarcom T., Iverson, B.L. and G. Georgiou, “E-clonal antibodies: selection of Full-Length IgG Antibodies             Using Bacterial Periplasmic Display” Nature Protocol. 3:1766-77 (2008) PMID 18948976.

Arredondo, S., Segatori L., Gilbert H.G. and G. Georgiou, “De novo Engineering and Evolution of Disulfide Isomerases Analogous to the Bacterial DsbC,” J. Biol. Chem 283(46):31469-76 PMCID: PMC2581571 (2008) pdf

Varadarajan, N., Cantor, JR, Georgiou G. and B.L. Iverson, “Engineering a Protease that Cleaves after Sulfotyrosine,” Angew. Chem Intl Ed. 47(41):7861-3 (2008) PMCID: PMC2671331 pdf

Skretas, G. and G. Georgiou, “Engineering G Protein-Coupled Receptor Expression in Bacteria, “ Proc Natl Acad Sci U S A. 105:14747-8. (2008). PMID: 18812517 pdf

Link, J.A., Skretas, G., Strauch, E.-M., Chari, N.S. and G. Georgiou, " Efficient Production of Membrane- Integrated and Detergent-Soluble G Protein-Coupled Receptors in Escherichia coli”  Protein Sci pdf  

Faulkner MJ, Veeravalli K, Gon S, Georgiou G, Beckwith J, "Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways," Proc Natl Acad Sci 105(18):6735-6740 (2008). pdf

Li, H., Hwang, B-Y., Lamikanthan, G., Blaber, S.I., Blaber, M., Golubkov, P. A., Ren, P., Iverson, B.L. and G. Georgiou, “The Substrate Specificity of Kallikrein 1 and 6 Determined by Phage Display,” Prot Sci  17(4):664-72 (2008). pdf

Cobaugh, C.W., M.S.; Almagro, J.C., Pogson, M., Iverson, B.L. and G. Georgiou, “Synthetic Antibody Libraries Focused Towards Peptide Ligands,” J. Mol. Bio 378(3):622-33 (2008) PMCID: PMC2494707 pdf

Varadarajan, N., Rodriguez S., Hwang, B-Y., Georgiou G. and B.L Iverson, “Highly active and selective endopeptidases with programmed substrate specificities,” Nature Chem. Biol.4:290-4 (2008). PMID: 18391948 pdf
              • News and Views Nature Chem. Bio 4:290-4 (2008).
            • Selected as a “Recommended” paper www.facultyof1000.com

Masip, L., Klein D., Quan S., Bardwell J.A.C. and G. Georgiou, “Laboratory Evolution of Escherichia coli thioredoxin for enhanced catalysis of protein oxidation in the periplasm reveals a phyolgentically conserved substrate specificity determinant” J. Bio. Chem. 283(2):840-8 (2008) PMID: 18003618 pdf

2007

Strauch E.-M. and G. Georgiou, “E. coli tatC mutations that suppress defective twin-arginine transporter signal peptides,” J. Mol Bio. 374:283-91 (2007) PMCID: PMC2661573  pdf

Link, J.A., Jeong, K-J. and G. Georgiou, “Beyond Toothpicks: New methods for isolating mutant bacteria ” Nature Rev. Microbiol. 5:680-8 (2007) PMID: 17676054 pdf

Jeong, K-J., Seo M-J., Iverson, B.L. and G. Georgiou, “APEx 2-hybrid, a quantitative protein-protein interaction assay for antibody discovery and engineering” Proc. Natl. Acad.. Sci. U.S.A 104;8247-52 (2007) PMCID: PMC1895937 pdf

Jung, S.T., Jeong, K.J., Iverson, B.L. and Georgiou, G. "Binding and Enrichment of Escherichia coli Spheroplasts Expressing Inner Membrane Tethered scFv Antibodies on Surface Immobilized Antigens" Biotechnol & Bioeng. 98:39-47 (2007)PMID: 17657772 pdf

Strauch E.-M and G. Georgiou, “A Bacterial Two-Hybrid System Based on the Twin Arginine Transporter Pathway of E.coli”  Protein Sci. 16:1001-8 (2007) PMCID: PMC2206650   pdf

Ribnicky, B., Van Blarcom, T. and G. Georgiou, “A scFv Antibody Mutant Isolated in a Genetic Screen for Improved Export via the Twin Arginine Transporter Pathway Exhibits Faster Folding,” J. Mol. Bio 369:631-9 (2007) PMCID: PMC1995598 pdf

Mazor, Y., Van Blarcom, T., Mabry, R., Iverson, B.L. and G. Georgiou, “Isolation of Engineered Full-Length Antibodies from Libraries Expressed in E. coli” Nature Biotechnology  25:563-5 (2007). PMID: 17435747pdf
                        • Nature 447:741-3 (2007);  News & Views in Nature Biotechnology 25:537-8 (2007); covered in numerous science news web sites
                        • Selected as a “Must Read” paper, www.facultyof1000.com

Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP. "Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1," Proc Natl Acad Sci U.S.A. 1;104(18):7379-84 (2007). pdf

Levy, R., Molineux, I., Iverson, B.L. and G. Georgiou, “Isolation of trans-Acting Genes that Enhance Soluble Expression of scFv Antibodies in the E.coli Cytoplasmby Lambda Phage Display,” J. Immunol. Meth.  10:164-173 (2007) PMID: 17328908 pdf

Tullman-Ercek D, DeLisa MP, Kawarasaki Y, Iranpour P, Ribnicky B, Palmer T, Georgiou G. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides" J Biol Chem 282:8309-16 (2007) PMID: 17218314 pdf

Hwang, B.Y., Varadarajan, N., Li, H., Rodriguez, S., Iverson, B.L. and Georgiou, G. "Substrate Specificity
of the Escherichia coli Outer Membrane Protease OmpP" J Bacteriol. 189:522-30 (2007) PMCID: PMC1797397 pdf

2006

Griswold, K.E., Aiyappan, N.S., Iverson, B.L. and G. Georgiou, "The Evolution of Catalytic Efficiency and Substrate Promiscuity in Human Theta Class 1-1 Glutathione Transferase"  J. Mol. Biol. 364:400-10 (2006) PMCID: PMC1995603 pdf

Gao, J., Lee, K., Zhao, M., Qiu, J., Zhan, X., A. Saxena, Cohen, S.N. and G. Georgiou, “Differential Modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome, ” Mol  Microbiol 61:394-406 (2006) PMID: 16771842 pdf

* Selected as a “Recommended” paper, www.facultyof1000.com

Lee, P.A., Tullman-Ercek, D. and G. Georgiou, “The Bacterial Twin Arginine Translocation Pathway” Ann Rev. Microbiol  60: 373-95 (2006) PMCID: PMC2654714 pdf

Mabry, R.,  Brasky R., Geiger, R., Carrion Jr. R.,  Hubbard, G.B., Leppla, S., Patterson, J.L., Georgiou, G.,  Iverson, B.L. “Detection of Anthrax Toxin in the Circulation of Animals Infected with Bacillus anthracis by using Engineered Immunoassays,” Clinical and Vaccine Immunol 13:671-6 (2006) PMCID: PMC1489546 pdf        

Masip, L., Veeravalli, K and G. Georgiou “The Many Faces of Glutathione,” Antiox. Redox Signal. 8:753-62 (2006 )PMID: 16771667 pdf

Sweeney, R.Y., Park, E.Y., Iverson B.L. and G. Georgiou “Assembly of MultimericPhage Nanostructures through Leucine Zipper Interactions” Biotechnol. & Bioeng 20:539-45 (2006) PMID: 16897782 pdf

Zhao, M., Kawarasaki, Y., Zhou L. and G. Georgiou, “Regulation of RraA, a Protein Inhibitor of RNase E-Mediated RNA Decay,” J. Bacteriol. 188:3257-63 (2006) PMCID: PMC1447450 pdf

Pothukuchy, A., Mano, N., Georgiou, G. and A. Heller “A potentially insect-implantable trehalose electrooxidizing anode,” Biosensors & Bioelectron. 22:678-84 (2006) PMID: 16546370 pdf

Harvey, B.R., Shanafelt, A.,  Baburina, I., Hui, R., Vitone, S., Iverson, B.L. and G. Georgiou, “Engineering of Recombinant Antibody  Fragments to Methamphetamine by Anchored Periplasmic Expression (APEx)” J. Immunol Methods 20:43-52 (2006) PMID: 16337958 pdf

Segatori, L., Murphy, L., Arredondo, S., Kadokura, H., Beckwith, J., Gilbert, G. and G. Georgiou, “Conserved Role of the a-helix linker of the Bacterial Disulfide Isomerase DsbC in the Avoidance of Misoxidation by DsbB,” J. Biol. Chem.  281:4911-9 (2006)PMID: 16280324 pdf

2004-2005

• Mabry, R., Rani, R., Geiger, R., Hubbard, R., Carrion, R., Brasky, K., Patterson, J., Georgiou, G. and         B.L. Iverson “High Affinity Antibody Fragments Lacking Fc Regions Confer Protection Against        Inhalation Anthrax,” Infect & Immun 73:8362-8 (2005). PMCID: PMC1307075
• Jeong, K.-J., Mabry, R. and G. Georgiou, “Avimers Hold their Own” Nature Biotechnol.23:493-4 (2005). PMID: 16333289
• Pressman,  J.G., Georgiou G., Speitel G. E. Jr. “Scale-up Considerations for a Hollow-Fiber-Membrane Bioreactor Treating Trichloroethylene-Contaminated Water,” Water Environ Res. 77:533-42 (2005). PMID: 16274088
• Drummond, D.A., Iverson, B.L., Georgiou, G. and Arnold, F.H., "Why High-error-rate Random Mutagenesis Libraries are Enriched in Functional and Improved Proteins" J. Mol. Biol. 350:806-16 (2005).
• Griswold, K.E., Kawarasaki, Y., Ghoneim, N., Benkovic, S.J., Iverson, B.L., and G. Georgiou, "Evolution of highly active enzymes by homology-independent recombination" Proc. Natl. Acad.. Sci. U.S.A. 102:10082-87 (2005).
• Sheoran, A.S., Chapman-Bonofiglio, S., Harvey, B.R., Mukherjee, J., Georgiou, G., Donohue-Rolfe, D. and S. Tzipori,  “Humanantibody against Shiga toxin 2 administered to piglets after the onset of diarrhea due to  Escherichia coli O157:H7 prevents fatal systemic complications,”  Infect. & Immun. 73:4607-13.
• Varadarajan, N., Gam J. Olsen, M.A., Georgiou, G. And B.L. Iverson, ”Engineering of Protease Variants Exhibiting High Catalytic Activity and Exquisite Substrate Specificity”  Proc. Natl. Acad.. Sci. U.S.A. 102:6855-60 (2005).
• Hicks, M., Lee, P.A., Georgiou, G., Berks, B.C. and T. Palmer, "Positive Selection for Loss-of-Function tat Mutations Identifies Critical Residues Required for TatA Activity" J. Bacteriol. 187:2920-5 (2005).
• Goldman, E.R., Medintz, I.L., Hayhurst, A., Anderson, G.P., Mauro, M., Iverson, B.L., Georgiou, G. and Mattoussi, H. “Luminescent Quantum Dot Bioconjugates Prepared Using Proteins Engineered with Histidine Tags” Analytica Chimica Acta 534:63-7 (2005).
• Lin, S.C. and G. Georgiou, “A Biocatalyst for the Removal of Sulfite from Alcoholic Beverages” Biotech & Bioeng., 89:123-127 (2005).
• Sweeney, R.Y., Mao, C., Gao, X., Burt, J.L., Belcher, A.M., Georgiou, G. and B.L. Iverson, “Bacterial Biosynthesis of Cadmium Sulfide Nanocrystals,” Chemistry & Biology 11:1553-9 (2004).  
• Jeong, K.J., Kawarasaki, Y., Gam, J.S., Harvey, B.R., Iverson, B.L. and Georgiou, G., “A Periplasmic Fluorescent Reporter Protein and its Application in High-throughput Membrane Protein Topology Analysis” J. Mol. Biol. 341:901-909 (2004). 
• Zhang, M., Monzingo, A.F., Segatori, L. , Georgiou, G. and J. D. Robertus, “The X-ray Structure of DsbC from Haemophilus influenzae” Acta Crystal. D 60:1512-8 (2004).
• McCarter, J.D., Stephens, D., Shoemaker, K., Rosenberg, S., Kirsch, J.F. and G. Georgiou, “Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpT” J. Bacteriol. 186:5919-25 (2004).
• Segatori, L. Gilbert H.G. and G. Georgiou, “Engineered DsbC Chimeras that Catalyze Both Protein Oxidation and Disulfide Bond Isomerization in E.coli: Reconciling Two Competing Pathways” Proc. Natl Acad. Sci. U.S.A. 101:10018-23 (2004). 
• Harvey, B., Georgiou G., Hayhurst, A., Jeong, K.J., Iverson, B.L. and G. Rogers, “Anchored Periplasmic Expression (APEx):   A Versatile Technology for the Isolation of High Affinity Antibodies from Combinatorial Libraries,”  Proc. Natl Acad. Sci. U.S.A. 101:9193-8 (2004). 
• Freeman, A., Cohen-Hadar, N., Abramov, S., Modai-Hod, R., Dror, Y and G. Georgiou,” Screening of a Million Cell Population by “Immobilized Cell on Immobilized Bead,” Approach,” Biotech & Bioeng 86:654-660 (2004).  
• Zhan X.,  Gao, J., Jain, C., Cieslewicz, M., Swartz, J.R. and  G. Georgiou, “Genetic Analysis of Disulfide Isomerization in E. coli:  The Expression of DsbC is Modulated by RNase E-Dependent mRNA  Processing,”    J.Bacteriol. 186:654-60 (2004).
• Masip, L., Pan, J., Haldar, S., Penner-Hahn, J.A., Delisa, M.P., Georgiou, G., Bardwell, J.C.A. and J.F. Collet, "Engineering a de novo Pathway for the Formation of Protein Disulfide Bonds in Bacteria," Science 303: 1185-1189 (2004).
• Mao, C., Solis, D.J., Reiss, B.D., Kottmann, S.T., Sweeney, R.Z., Hayhurst, A., Georgiou, G., Iverson, B.L. and A.M. Belcher,"Virus-Based Toolkit for the Directed Synthesis of Magnetic and Semiconducting Nanowires," Science 303: 213-17 (2004).
• DeLisa, M.P., Lee, P., Palmer, T. and G. Georgiou, ”Phage Shock Protein A (PspA) Relieves the Saturation of Protein Export Via the Tat Pathway,” J. Bacteriol 186:366-73 (2004).