- Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “"Physics-Based, Experimentally Validated Structural Ensemble of an Intrinsically Disordered Polypeptide" JACS (submitted)
- Saggy, I., Wine, Y., Georgiou G. and Benhar, I., “Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining” PEDS (2012)
- Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, “De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy,” ACS Chem. Bio. (2012)
- Wine, Y. Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M.,, Ellington,
A.D., Marcotte, E.M. and G. Georgiou, “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA (submitted)
- Li W., Cantor, J., Yogesha, S.D., Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M., and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation" ACS Chem. Bio. (2012)
- Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation
in the Structure of Human IgG Fc" ACS Chem. Bio (2012) PMID: 22747430
- Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T., Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV” J. Virology 86:9113-21 (2012) PMID: 2269665
- Skretas, G. Makino, T. Varadarajan N., Pogson M. and G. Georgiou, “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coli” Metabolic Eng. 14:591-602 (2012) PMID: 22609824
- Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E., Transl. Oncology 5:26-31 (2012) PMID: 22348173
- Ippolito, G.C., Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One 7:e35497 (2012) PMID: 2255816
- Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington, “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,” Chem. & Biol. 19:449-55 (2012) PMID: 22520751
- Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou, "Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2" ChemBiochem 3:649-53(2012) PMID: 22334509
- Cantor, J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno--genicity Enzymes for Amino Acid Depletion Therapy in Cancer,” Methods Enzymol. 502:291-312 (2012). PMID:22208990
- Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P.,
Chanhee,
J.
Stone, E.M., Georgiou, G. and Arthur E. Frankel, " Cytotoxicity of
Human Recombinant Arginase I [Co] PEG5000 in the Presence of
L-Citruline is Dependent on Arginosuccinate Synthetase Expression in
Human Cells"Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999
- Stone, E. M., Chantrranupong L., Gonzalez, C.,
O’Neal, J.,
Rani, M., VanDenBerg, C. and G. Georgiou, "Strategies for Optimizing
the Serum Persistence of Engineered Human Arginase I for Cancer
Therapy," J. Control. Release (accepted).
- Reddy S.T. and G. Georgiou, "Systems Analysis of
Adaptive
Immunity by Utilization of High Throughput Technologies," Curr
Opin.Biotechnol. 22: 584-9 PMID:21570821.
- Makino T. Skretas, G. and G. Georgiou, "Strain
Engineering
for
Improved Expression of Recombinant Proteins In Bacteria," Microbial
Cell Factories 10:32 PMID:21569582
- Feeney, M.A., Veeravalli, K., Boyd, D., Gon, S.,
Faulkner,
M.J.,
Georgiou, G. & Beckwith. J., "Rewiring MajorElectron Flow
Pathways in E.coli
by a Single Amino Acid Change in Lipoamide
Dehydrogenase" Proc. Natl Acad Sci USA 108:7991-6.
(2011) PMID:21521794
- Jung, S.T., Kang, T.H., Kelton, W. and G. Georgiou,
"Bypassing
Glycosylation: Engineered Aglycosylated Full Length IgG for Human
Therapy," Curr Opin.Biotechnol. (in press)
PMID:21420850
- Cantor J., Stone, E.M. and G. Georgiou, "Expression
and
Biochemical
Characterization of the Human Enzyme N-Terminal Asparagine
Amidohydrolase (hNTAN1)" Biochemistry 50:3025-33
(2011). PMID:21375249
- Cantor J., Yoo, T.H., Dixit, A., Iverson, B.L.,
Forsthuber,
T.
and G,
Georgiou "Therapeutic Enzyme Deimmunization by Combinatorial T-Cell
Epitope Removal Using Neutral Drift" Proc. Natl. Acad. Sci.
USA
108:1272-77 (2011). PMID:21209329
- Veeravalli, K., Boyd, D., Iverson, B.L., Beckwith, J.
and
G.
Georgiou,
"Laboratory Evolution of Glutathione Biosynthesis Reveals Natural
Compensatory Pathways," Nature Chem. Biol. 7:101-105
(2011) PMID:
21186348.
- Stone, E.M., Chantranupong, L., and G. Georgiou, "The
Second
Shell
Metal Ligands of Human Arginase Affect Coordination of Nucleophile and
Substrate" Biochemistry 49:10582-8
(2010) PMID: 21053939
- Makino, T., Skretas, G., Kang, T.-H. and G. Georgiou,
"Comprehensive
engineering of Escherichia
coli for enhanced expression of IgG
antibodies," Metabolic Eng. 13: 241-51 (2010)
PMID:21130896
- Reddy, S.T., Ge, X., Miklos, A.E., Hughes, R.A. Kang.
S-H.,
Hoi, K.-H.,
Chrysostomou. C., Hunicke-Smith, S., Iverson, B.L., Tucker, P.W.,
Ellington, A.D. & G. Georgiou, " Isolation of Monoclonal
Antibodies Without Screening by Mining the Variable Gene Repertoire of
Plasma Cells," Nature Biotechnol. 28:965-9
(2010). PMID: 20802495
• Leading Edge Select: Cell 143:491
(2010)
• "Better Antibodies Through Sequencing" Biotechniques
http://www.biotechniques.com/news/Better-monoclonals-through-sequencing/biotechniques-303792.html
- Qiu, J.K., Jung, S.T., Georgiou, G. and H.Y. Hang,
"Enrichment
of
Escherichia
coli
Spheroplasts Displaying scFv Antibodies Specific for
Antigens Expressed on the Human Cell Surface," Appl Microbiol
Biotechnol. 88:1385-91 (2010) PMID:
20878322
- Skretas G. and G. Georgiou, "A Simple Genetic
Selection for
the
Isolation of Overexpressed Genes that Enhance the Accumulation of
Membrane-Integrated Human G Protein-Coupled Receptors in Escherichia
coli" Appl. Environ. Microbiol 76:5852-9
(2010) PMID: 20639362
- Jung S.-T., Kang T.-H., and G. Georgiou "Efficient
Expression
and
Purification of Human Aglycosylated Fcγ receptors in Escherichia coli,"
Biotechnology & Bioengineering 107:21-30
(2010) PMID: 20506277
- Mazor, Y., Van Blarcom, T., Carroll, S., and G.
Georgiou,
"Selection of
full-length IgGs by tandem display on filamentous phage particles and
Escherichia
coli
fluorescence-activated cell sorting screening," FEBS
J. 277:2291-303 (2010) PMID: 20423457
- Ge, X., Mazor, Y., Hunicke-Smith S. P. Ellington A.D.
and
G.
Georgiou,
"Rapid Construction and Characterization of Synthetic DNA libraries
Without DNA Amplification," Biotechnology &
Bioengineering
106:347-57 (2010) PMID: 20198660
- Van Blarcom T, Sofa-Podesta, C., Ang J., Boyer, J.L.,
Crystal,
R. and G. Georgiou "Affinity Maturation of an
Anti-V Antigen IgG Expressed In Situ Via Adenovirus Gene Delivery
Confers Enhanced Protection Against Yersinia pestis
Challenge" Gene
Therapy 17:913-21 (2010) PMID: 20393511
- Stone, E.M., Chantranupong, L, Glazer S.E, Cherukuri,
P.,
Breece, R.M., Tierney D.L., Curley S.A. and G. Georgiou, "Replacing Mn+2
with Co+2 in Human Arginase I Enhances
Cytotoxicity Towards L-Arginine Auxotrophic Cancer Cell Lines" ACS
Chemical Biology 5:333-42 (2010) PMID:
20050660
- Jung, S.T., Reddy, S., Kang, T.H, Borrok, J.M.,
Sandlie,
I.,
Tucker, P.W. and G. Georgiou, "Aglycosylated IgG Variants Expressed in E.
coli that Selectively Bind FcγRI
Potentiate Tumor Cell Killing by Monocyte-Derived Dendritic Cells," Proc
Natl Acad Sci U S A 107:604-9 (2010)
PMID: 20080725
- Research Highlight: Nature Biotechnol.
28:45
(2010)
- Commentary by Osherovich, L. SciBX
3
(2010) doi:10.1038
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